Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.

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1,156 – 1,170 of 1,201 results

1,156

New England Biolabs, Inc. Nb.BsrDI – 1000 units

A restriction endonuclease that recognizes the sequence GCAATG_NN.

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1,157

New England Biolabs, Inc. T7 Endonuclease I – 1250 units

T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5' to the mismatch. The protein is the product of T7 gene 3.

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1,158

New England Biolabs, Inc. ScaI-HF – 1000 units

A high fidelity restriction endonuclease that recognizes the sequence AGT^ACT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

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1,159

Supply Solutions Sigma Aldrich - Phosphatase,alkaline(AP),MB Grade(1U/l) * Third Party Item

Sigma Aldrich - Phosphatase,alkaline(AP),MB Grade(1U/l) * Third Party Item

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1,160

Components	10X OPTIZYME™ Buffer 4
Cut Site	T.CTAGA
Form	Liquid
Product Type	XbaI
Storage Buffer	10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
For Use With (Application)	Indicates ligation efficiency of DNA fragments generated by digestion with the Restriction Enzyme

1,161

OPTIZYME™ HindIII, Fisher BioReagents™

Pricing & Availability
Specifications

Components	25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
Cut Site	A.AGCTT
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	HindIII
Storage Buffer	10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Incubator Temperature	37°C
Concentration	10 U/μL
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII

1,162

OPTIZYME™ AloI, Fisher BioReagents™

Pricing & Availability

1,163

OPTIZYME™ NotI, Fisher BioReagents™

Pricing & Availability
Specifications

Components	25 to 50% Water, >50% Glycerin
Cut Site	GC.GGCCGC
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	NotI
Storage Buffer	20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Concentration	10 U/μL
For Use With (Application)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I

1,164

Fisher Scientific OPTIZYME™ RsaI, Fisher BioReagents™

Pricing & Availability

1,165

OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™

Pricing & Availability
Specifications

Components	25 to 50% Water, >50% Glycerin
Cut Site	C.TNAG
Content And Storage	Keep container tightly closed
Form	Liquid
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
Concentration	10 U/μL
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI